EasySpiral Dilute®

High technology

Key features

Automatic serial diluter & plater with full traceability

1. WORLD PREMIERE: ALL-IN-ONE DILUTER AND PLATER
Save time: All-in-one dilution up to 10^-5 and automatic plating (no more hours of dispensing tubes, autoclaving and washing tubes, no more pipette calibration)
Save consumables: no more tips, reagents, Petri dishes
User-friendly: one button touch for 10^-5 serial dilution and plating
Make as many dilutions as you want
Increased accuracy : typical accuracy 0,5%

2. SO MUCH QUICKER !
5 automatic dilutions in 90 sec.
3 preset automatic plating modes
Automatic standardized plating of 30 to 10 millions CFU/mL on 1 Petri dish
25 seconds for a full cycle (disinfection, sample-taking and plating)
10 Petri dishes plated in less than 2 minutes (with the same sample)
Revolutionary rotating arm with high-speed movement (Patented)

3. NO CROSS-CONTAMINATION
Stylus disinfection system: inside and outside by overflow, 8 bars pressure (Patented)
Unique patented system by overflow
600 cleanings without changing bottles

4. TRACEABILITY
Increased repeatability within a team
Easy PC connectivity via USB
From 10 to 1000 microliters programmable volumes
Monitoring software with traceability

5. EXCELLENT REPRODUCIBILITY AND REPEATABILITY
In compliance with AFNOR V08-100 / ISO 7218

Technical specifications

easySpiral^® Dilute
Automatic sample diluter & plater

Controlled by microprocessor
Petri dish diameter: 50 mm, 90 mm and 150 mm
Syringe capacity: 1000 micro liters
Dispensed volume: from 10 to 1000 microliters

Preset volume dispensed: 50, 100 and 200 microliters
Detection in CFU/ml: 30 to 10 millions CFU/mL

Plating modes:
Constant (Homogeneous deposition): 50 microliters, 100 microliters, 200 microliters and USB programmable volumes
Exponential (Decreasing concentration): 50 microliters, 100 microliters, 200 microliters and USB programmable volumes
Circle mode (3 dilutions on one 90 mm Petri dish): 10 microliters inner circle, 16 microliters middle circle and 25 microliters outer circle.

Number of dilutions at 1/10th for 1 cycle: 5
Full dilution cycle: 90 sec.
Full plating cycle: 25 sec.
Full cycle (Disinfection, 5 dilutions, plating of 6 dishes): 3 min. 40 sec.

Programmable volume by USB: yes
Filling time programmable by USB (for viscous samples) : yes
Sample mixing before dilution / plating

Traceability: Excel™ export
Patented circle mode: 3 dilutions on one 90 mm Petri dish / 6 dilutions on one 150 mm Petri dish

Diluent autonomy: 55 cycles of 5 dilutions (with 2L bottles)
Stylus disinfection system: inside and outside by overflow, 8 bars pressure (Patented)
Overflow technology: 8 bars pressure
Monthly cleaning program
Cleaning capacity: 600 cycles (2L bottles)

Successive plating capacity with the same sample: 10 Petri dishes (50 microliters)

Delivered with: 1000 sterile beakers, 1 blue dye for testing, 1 liquid detergent, 3 sets of connectors for autoclave, 90 mm Spiral counting grid, 150 mm Spiral counting grid , 150 mm Petri dish ring , 55 mm Petri dish ring, software on a Cd-Rom, USB cable, power cable, user manual.

Option : 2L GL45 bottles, Housing protection, Barcode reader

Dimensions: 39 x 42 x 29 cm
Weight: 15.6 kg
Power supply: 100-240 V / 50 Hz to 60 Hz

1 year guarantee (after registration)

Ref. 414 000

Options


Housing protection for working outside laminar flow	Maintenance kit (1 stylus + 1 syringe + 1 silicone tubing kit + 1 grease tube)	Beakers DB50, sterile beakers
Ref. 413 001	Ref. 413 007	Ref. 415 100


Connection for GL 45 bottles	2 L Bottle	Barcode reader
Ref. 413 003	Ref. 413 002	Ref. 522 000

Multi adapable support for Petri dishes from 55 mm to 65 mm

Ref. 413 019

F.A.Q

Q: Is easySpiral^® Dilute innovative ?
A: Yes ! easySpiral^® Dilute is the only 2-in-1 equipment making automatic serial dilutions and standardized plating. With the one-button touch for the routine serial dilutions and automatic plating, EasySpiral^® Dilute enables serial dilutions up to 10^-5CFU/mL with typical accuracy of 0,5%. It then enables automatic standardized plating of 30 to 10 millions countable CFU/mL on 1 single Petri dish.

Q: Can I use peptone water as diluant?
A: No, because all diluants with nutriments can result in contamination of the machine and of the sample. You can use all saline solutions, for exampleringer solution or saline solution. As the bacterias stays in the beakers for only a few seconds before going on a nutriment solution on the agar, the dilution with only a saline solution has no consequence on the overall count.

Q: EasySpiral Dilute can only dilute up to 10^-5. I do 10^-7. Do I need to do 2 dilutions by hand?
A: No, you can do a 10-5 dilution, take the last beaker and replace it as the mother sample and then proceed with the automatic dilution.

Q: I usually use 9 mL tubes and 1 mL sample...
A: Common precision for the filling of the 9 mL tubes is 2% and pipettes precision for the 1 mL intake is 2%, and they must be often calibrated and checked. The EasySpiral dilute has a high precision Hamilton glass syringe, with no air displacement, which allows 0.5 % precision and accuracy on 4.5 mL and 0.5 mL volumes. Conclusion, you can obtain much higher precision with volumes cut by half!

Q: The accuracy of EasySpiral plater is high: 0.5%. How can I check it?
A: There is a special test procedure which fills a beaker with 0.5 mL ans another one with 4.5 mL. you can then weight then to check the machine.

Q: What is automatic plating ?
A: When sample preparation is over, Petri dishes are plated to perform the microbiological analysis. easySpiral^® is an automatic plater that allow to have all the successive dilution on one Petri dish, avoiding all the tedious stages of dilutions and successive incubations of the manual method.

Is the Spiral^® method an official method?
A: Yes, it is validated by A.O.A.C (American Organization of Anlysis Control) and AFNOR (Association Française de Normalisation), V 08-100 standard. It became one of the official methods for food and cosmetics applications. The Spiral^® method quickly became a reference for food microbiology applications, bactericidal, medical and cosmetical researches. It has been validated by many private analytical laboratories, veterinary laboratories and international industrial groups.

What are Spiral^®’s advantages ?
A: They are numerous:
It is a repeatable and reproducible method that performs all the dilutions on one Petri dish (compared to the three -minimum- that are necessary with the manual method).
The sensitivity goes from 30 to 10 million CFU / mL on a Petri dish without prior to sample dilution (4 Petri dishes are needed in the manual method).
It is an automatic method (intaking, plating, disinfection and cleaning) which makes these operations economical in consumables, time and space, and ecological.
No cross-contamination, regularity and standardization, it is a time and consumables saving process.

When was the Spiral^® method invented ?
A: The Spiral^® method invented in 1973 when Dr Ed Campbell, a researcher at the FDA (US Food and Drug Administration) designed a method to automatize the routine work of the bacterial enumeration. With François Jalenques, physicist and founder of INTERSCIENCE, they developped this innovative process. The Spiral® method was updated and patented in 1992. Today Spiral^® automatic platers guarantee quick and safer ananlyses.

Does easySpiral^® require a lot of maintenance ?
A: No, it’s a device that requires only little maintenance because it is robust and stainless steel. Thanks to its design, the circuit board is protected from liquid projections.